Evaluation of real time PCR assays for the detection and enumeration of enterohemorrhagic Escherichia coli directly from cattle feces.

Evaluation of real time PCR assays for the detection and enumeration of enterohemorrhagic <i>Escherichia coli</i> directly from cattle feces

Evaluation of ehxA, stx1, and stx2 Virulence Gene Prevalence in Cattle Escherichia coli Isolates by Multiplex PCR

  Today, it is nearly 25 years past from investigation of Shiga toxigenic Escherichia coli (STEC) which is able to produce Shiga toxins and cause different gastroenteritis. Since incidence of gastroenteritis due to STEC is increasing, it's necessary to develop rapid, specific and accurate procedures like PCR. In this study, we used PCR method to detect and identify STEC in cultures of 55 Escher...

PCR detection of Escherichia coli O157:H7 directed from slaughtered cattle in Shiraz, Iran

  Escheric hia coli O157:H7 lives in the intestines of healthy cattle, and can contaminate meat during slaughtering pr actices . Detection of the low infectious dosage of bacterium requires a sensitive method. We developed polymerase chain reaction (PCR) assays to detect the gene Stx2 irrespective of the bacterial serotype. In this study, the detection limit of the PCR protocol in detecting Stx...

Detection of Colicin genes by PCR in Escherichia coli isolated from cattle in Shiraz-Iran

A variety of probiotic bacteria have been tested to control animal and foodborne pathogenic bacteria in livestock. The mechanism of inhibition of pathogenic bacteria for several of those probiotic microorganisms is mediated by the production of bacteriocins. Colicins are probably the group of bacteriocins that have been most thoroughly characterized. Colicins are antimicrobial proteins produced...

Molecular investigation of the prevalence and detection of enterohemorrhagic Escherichia coli isolated from traditional cheese in Iran

Introduction: Shiga toxin-producing Escherichia coli (STEC) strains are one of the main causes of food poisoning and diseases such as hemorrhagic colitis and hemolytic uremic syndrome.  Food contamination mainly occurs through food of animal origin such as meat and dairy products. The aim of this study was to investigate prevalence and distribution of virulence genes in Escherichia coli isolate...